Product Name :
Phospho-IRE1 (S724) Recombinant Rabbit Monoclonal Antibody [PSH03-35]
Predicted band size :
110 kDa
Observed band size :
110 kDa
Synonyms:
Endoplasmic reticulum (ER) to nucleus signalling 1 antibody Endoplasmic reticulum to nucleus signaling 1 antibody Endoplasmic reticulum-to-nucleus signaling 1 antibody Endoribonuclease antibody ER to nucleus signaling 1 antibody ERN 1 antibody Ern1 antibody ERN1_HUMAN antibody hIRE 1p antibody hIRE1p antibody 展开
Function :
The serine/threonine-protein kinase/endoribonuclease inositol-requiring enzyme 1 α (IRE1α) is an enzyme that in humans is encoded by the ERN1 gene.6-Chloro-5H-benzo[a]phenoxazin-5-one Chemical name The protein encoded by this gene is the ER to nucleus signalling 1 protein, a human homologue of the yeast Ire1 gene product.Bis(2-(2-methoxyethoxy)ethyl)amine Data Sheet This protein possesses intrinsic kinase activity and an endoribonuclease activity and it is important in altering gene expression as a response to endoplasmic reticulum-based stress signals (mainly the unfolded protein response). Two alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Antibody Type:
Recombinant Rabbit monoclonal Antibody
Immunogen :
Synthetic phospho-peptide corresponding to residues surrounding Ser724 of human IRE1.
Species reactivity:
Human, Mouse
Verify application:
WB, IF-Cell, IHC-P, FC
Coupling :
unconjugated
Clone number:
PSH03-35
RRID:
AB_3096844
Form :
Liquid
Concentration :
1ug/ul
Storage instructions:
Store at +4℃ after thawing.PMID:33739412 Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer solution:
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Subtype:
IgG
Purification method :
Protein A affinity purified.
ELTSA :
Molecular weight :
Predicted band size: 110 kDa
Positive control :
HeLa starved for 3 hours then treated with 100nM Calyculin A for 30 minutes cell lysate, Jurkat treated with 100nM Calyculin A for 30 minutes cell lysate, Jurkat cells treated with 100nM Calyculin A for 30 minutes, human pancreas tissue, HeLa cells treated with 100nM Calyculin A for 30 minutes.